Construction and Characterization of a cDNA Library from Wheat Infected with Fusarium graminearum Fg 2

Total RNA from wheat spikes infected with F. graminearum Fg2 was extracted and the mRNA was purified. Switching Mechanism at 5′ end of the RNA Transcript (SMART) technique and CDS Ill/3′ primer were used for first-strand cDNA synthesis using reverse transcriptase by RT-PCR. Primer extension polymerase chain reaction was used to construct the double-strand cDNA that was digested by proteinase K, then by Sfi I and fractionated. cDNAs longer than 0.5 kb were collected and ligated to λTriplEx2 vector followed λ phage packaging reaction and library amplification. The qualities of both unamplified and amplified cDNA libraries were strictly checked by conventional titer determination. One hundred and sixty five plaques were randomly picked and tested using PCR with universal primers derived from the sequence flanking the vector. A high quality cDNA library from wheat spikes that have been infected by F. graminearum was successfully constructed

Chandra Detects the Rare Oxygen-type Wolf-Rayet Star WR 142 and OB Stars in Berkeley 87

We present first results of a Chandra X-ray observation of the rare oxygen-type Wolf-Rayet star WR 142 (= Sand 5 = St 3) harbored in the young, heavily-obscured cluster Berkeley 87. Oxygen type WO stars are thought to be the most evolved of the WRs and progenitors of supernovae or gamma ray bursts. As part of an X-ray survey of supposedly single Wolf-Rayet stars, we observed WR 142 and the surrounding Berkeley 87 region with Chandra ACIS-I. We detect WR 142 as a faint, yet extremely hard X-ray source. Due to weak emission, its nature as a thermal or nonthermal emitter is unclear and thus we discuss several emission mechanisms. Additionally, we report seven detections and eight non-detections by Chandra of massive OB stars in Berkeley 87, two of which are bright yet soft X-ray sources whose spectra provide a dramatic contrast to the hard emission from WR 142.Comment: To appear in the Astrophysical Journa

Lower bounds for several online variants of bin packing

We consider several previously studied online variants of bin packing and prove new and improved lower bounds on the asymptotic competitive ratios for them. For that, we use a method of fully adaptive constructions. In particular, we improve the lower bound for the asymptotic competitive ratio of online square packing significantly, raising it from roughly 1.68 to above 1.75.Comment: WAOA 201

High pressure mechanical seal

A relatively impervious mechanical seal is formed between the outer surface of a tube and the inside surface of a mechanical fitting of a high pressure fluid or hydraulic system by applying a very thin soft metal layer onto the outer surface of the hard metal tube and/or inner surface of the hard metal fitting, prior to swaging the fitting onto the tube. The thickness of such thin metal layer is independent of the size of the tube and/or fittings. Many metals and alloys of those metals exhibit the requisite softness, including silver, gold, nickel, tin, platinum, indium, rhodium and cadmium. Suitably, the coating is about 0.0025 millimeters (0.10 mils) in thickness. After swaging, the tube and fitting combination exhibits very low leak rates on the order or 10.sup.-8 cubic centimeters per second or less as meaured using the Helium leak test

X-ray Variability in the Young Massive Triple theta2 Ori A

Massive stars rarely show intrinsic X-ray variability. The only O-stars credited to be intrinsically variable are theta1 Ori C due to effects from magnetic confinement of its wind, and theta2 Ori A suspected of similar activity. Early Chandra observations have shown that the most massive star system in the Orion Trapezium Cluster, theta2 Ori A, shows rapid variability on time scales of hours. We determine X-ray fluxes and find that the star shows very strong variability over the last 5 years. We observed a second large X-ray outburst in November 2004 with the high resolution transmission grating spectrometer on-board Chandra. In the low state X-ray emissivities indicate temperatures well above 25 MK. In the high state we find an extended emissivity distribution with high emissivities in the range from 3 MK to over 100 MK. The outburst event in stellar terms is one of the most powerful ever observed and the most energetic one in the ONC with a lower total energy limit of 1.5x10^37 ergs. The line diagnostics show that under the assumption that the line emitting regions in the low states are as close as within 1 -- 2 stellar radii from the O-star's photosphere, whereas the hard states suggest a distance of 3 -- 5 stellar radii. The two outbursts are very close to the periastron passage of the stars. We argue that the high X-ray states are possibly the result of reconnection events from magnetic interactions of the primary and secondary stars of the spectroscopic binary. Effects from wind collisions seem unlikely for this system. The low state emissivity and R-ratios strengthen the predicament that the X-ray emission is enhanced by magnetic confinement of the primary wind. We also detect Fe fluorescence indicative of the existence of substantial amounts of neutral Fe in the vicinity of the X-ray emission.Comment: 11 pages, 8 figures, accepted for publication in The Astrophysical Main Journa

Focusing by blocking: repeatedly generating central density peaks in self-propelled particle systems by exploiting diffusive processes

Over the past few years the displacement statistics of self-propelled particles has been intensely studied, revealing their long-time diffusive behavior. Here, we demonstrate that a concerted combination of boundary conditions and switching on and off the self-propelling drive can generate and afterwards arbitrarily often restore a non-stationary centered peak in their spatial distribution. This corresponds to a partial reversibility of their statistical behavior, in opposition to the above-mentioned long-time diffusive nature. Interestingly, it is a diffusive process that mediates and makes possible this procedure. It should be straightforward to verify our predictions in a real experimental system.Comment: 6 pages, 6 figure

An Atlas of K-line Spectra for Cool Magnetic CP Stars: The Wing-Nib Anomaly (WNA)

We present a short atlas illustrating the unusual Ca {\sc ii} K-line profiles in upper main sequence stars with anomalous abundances. Slopes of the profiles for 10 cool, magnetic chemically peculiar (CP) stars change abruptly at the very core, forming a deep "nib." The nibs show the same or nearly the same radial velocity as the other atomic lines. The near wings are generally more shallow than in normal stars. In three magnetic CP stars, the K-lines are too weak to show this shape, though the nibs themselves are arguably present. The Ca {\sc ii} H-lines also show deep nibs, but the profiles are complicated by the nearby, strong H$\epsilon$ absorption. The K-line structure is nearly unchanged with phase in $\beta$ CrB and $\alpha$ Cir. Calculations, including NLTE, show that other possibilities in addition to chemical stratification may yield nib-like cores.Comment: 6 pages, 2 figures, and 8 figures; accepted for publication in ApJ

Color LAR codec : a color image representation and compression scheme based on local resolution adjustment and on self-extracting region representation

This LAR (Locally Adaptive Resolution) color image coding scheme yields to an efficient progressive compression with a better subjective quality than Jpeg2000. Additionally, it offers region functionalities for low bit rate coding and decoding. From highly compressed luminance, a region description, without contours encoding, can be obtained through a segmentation process performed at both coder and decoder. Considering color results, controlled chrominance components segmentation provides a better data consistency simultaneously with a low bit rate compression. As regions and their encoding are based on a same representation grid, enhancement of image quality can be global, or only restricted to a Region Of Interest.Cet article présente un schéma original de codage progressif d'images couleur apportant à la fois une efficacité en termes de compression (meilleure qualité subjective que Jpeg2000) et des fonctionnalités au niveau région à bas débits pour le codeur et le décodeur. À partir de l'image des luminances codée à bas débit par le codec LAR (Locally Adaptive Resolution), une description en régions, sans codage des contours, est obtenue à travers un procédé de segmentation effectué au codeur et au décodeur. Cette segmentation peut être contrôlée par les composantes chromatiques pour une meilleure cohérence du résultat d'un point de vue couleur. Un codage basé régions appliqué sur les images de chrominance produit alors une compression de ces composantes à très bas débit. Comme les régions et le codage de leur contenu partagent une même grille de représentation, l'amélioration de la qualité de l'image peut être globale, ou limitée à une zone d'intérêt

Predictions for mass-loss rates and terminal wind velocities of massive O-type stars

Mass loss forms an important aspect of the evolution of massive stars, as well as for the enrichment of the surrounding ISM. Our goal is to predict accurate mass-loss rates and terminal wind velocities. These quantities can be compared to empirical values, thereby testing radiation-driven wind models. One specific issue is that of the "weak-wind problem", where empirically derived mass-loss rates fall orders of magnitude short of predicted values. We employ an established Monte Carlo model and a recently suggested new line acceleration formalism to solve the wind dynamics consistently. We provide a new grid of mass-loss rates and terminal wind velocities of O stars, and compare the values to empirical results. Our models fail to provide mass-loss rates for main-sequence stars below a luminosity of log(L/Lsun) = 5.2, where we run into a fundamental limit. At luminosities below this critical value there is insufficient momentum transferred in the region below the sonic point to kick-start the acceleration. This problem occurs at the location of the onset of the weak-wind problem. For O dwarfs, the boundary between being able to start a wind, and failing to do so, is at spectral type O6/O6.5. The direct cause of this failure is a combination of the lower luminosity and a lack of Fe V lines at the wind base. This might indicate that another mechanism is required to provide the necessary driving to initiate the wind. For stars more luminous than log(L/Lsun) = 5.2, our new mass-loss rates are in excellent agreement with the mass-loss prescription by Vink et al. 2000. This implies that the main assumption entering the method of the Vink et al. prescriptions - i.e. that the momentum equation is not explicitly solved for - does not compromise the reliability of the Vink et al. results for this part of parameter space (Abridged).Comment: 10 pages, 10 figures, Astronomy & Astrophysics (in press